Container disinfection according to European standard DIN EN 17272

The standardised, safe and validatable state-of-the-art hydrogen peroxide container decontamination !

Background: Validatable container preparation with reproducible disinfection quality

We protect your valuable containers including container cargo from dangerous pathogens according to the current state of disinfection technology - and with a fast reaction time of only 60 minutes!

Sea freight containers and, in general, all types of logistics containers that are shipped around the globe every day are naturally exposed to a greater or lesser degree of environmental and surface contamination by biological agents. Accordingly, disinfecting reprocessing of such containers is a demanding and time-consuming task. After all, people, animals and products that come into contact with these containers should be hygienically protected in the best possible way - especially in the global age of pandemics, epidemics, diseases and the like.

Our question therefore: Do you already know the DIOP disinfection method confirmed according to European disinfection standard DIN EN 17272 for your demanding container fumigations / container fogging against bacteria, fungi, viruses and spores?

Disinfect container

A real milestone for your container decontamination, your container hygiene management as well as your automated validated container disinfection.

In order to give your container port and container terminal more security through standardisation in the increasing number of disinfection procedures and systems for automated room, air and surface disinfection, the EU adopted the new DIN EN 17272 specification as the new state of the art in mid-2020. In the area of validatable final disinfections, this is also used for the seamless reprocessing of shipping containers.

As a disinfector or hygiene manager in your container terminal and port, make sure that you safeguard your conventional, manual disinfection methods with standardised disinfection procedures with reproducible disinfection quality .

DIN EN 17272 Testing

Important for the automated reprocessing of containers according to the European Disinfection Standard

A standardised disinfection process (EN 17272), tested according to European standards, gives you the microbiological, technical and legal possibility to carry out automated, validated high-level disinfection in all critical environments, including containers, hazardous goods containers and even vehicles. 

3 disinfection solutions tailored to your container hygiene and container safety needs

1. mobile, portable multifunctional fogging devices for your complete container disinfection

Automated and validatable final disinfection for your container management through reproducible disinfection quality

Traditional surface disinfection methods in container environments (e.g. mechanical scrub-wipe disinfection or spray disinfection) have limited effectiveness because they rely on the operator to ensure proper surface selection, product preparation and disinfectant contact time. In contrast, rolling or portable "no-touch" room disinfection machines (H2O2 aerosol generators) reduce operator dependency and can thus significantly improve the effectiveness of final disinfection of containers and transport vehicles of all types.

In addition, you compensate for the deficits of non-validatable, manual cleaning and disinfection processes (risk minimisation through microbiological container validation).

In such a final disinfection, every square centimetre of a container must be disinfected. This often does not happen and deficits occur, which the automated nebulisation according to DIN EN 17272 compensates for.

What types of containers can you conveniently have them refurbished?

  • Shipping container / ISO container for sea freight (sea freight container)
  • Refrigerated container (reefer) / integral container
  • Laboratory container
  • Storage container
  • Office container
  • Sanitary containers with showers, toilets, urinals etc.
  • High Cube Container (HC)
  • Container systems for military, fire brigades and disaster control
  • Bulk containers, multi-purpose containers, platform containers and much more.

Included scope of services for your port or container port terminal

  • Your DiosolGenerator model for automated container decontamination: MF Professional
  • Starter package for H2O2 generator incl. diosol biocide, chemo indicators, multifunctional accessories etc.
  • Instruction training at your port terminal on site
  • Certification by a state-certified disinfector
  • DIN EN 17272 disinfection certificate including validation documents, microbiological reports and sample templates for operating instructions, SOPs, etc. 
H2O2 nebuliser

The stress-free solution for your seamless and validated reprocessing of shipping containers according to the current state of disinfection technology

2. mobile multifunctional disinfection systems on wheels for your H2O2 container decontamination.

Automated and validatable final disinfection for rooms of all types, sizes and complexities through reproducible disinfection quality

Just as with sophisticated vehicle preparation, all objects in rooms that are touched by many different people are potentially contaminated with all kinds of pathogens (e.g. noro and coronaviruses, mycobacteria / tuberculosis pathogens, multi-resistant bacteria, moulds, clostridia spores, etc.).

In contrast to manual disinfection work or physical disinfection with UV light as well as dangerous ozonisation, you can also reach corners, edges, crevices and angles that are difficult and time-consuming to reach with disinfectant wipes by means of environmentally friendly hydrogen peroxide cold fogging. 

Especially in rooms with technical installations or in your emergency vehicles, there are a large number of switches that must not be moved by mistake. By means of gapless H2O2 fogging, these contaminated and neglected zones are disinfected safely and without contact.

Scope of services included

  • Your DiosolGenerator model for mobile container preparation: PROTEC TUBE+
  • Starter package for H2O2 generator incl. diosol biocide, chemo indicators, multifunctional accessories etc.
  • Instruction training at your port terminal on site
  • Certification by a state-certified disinfector
  • DIN EN 17272 hygiene certificate with all important documents and records for your hygiene and disinfection plan
Cold nebuliser H2O2

The carefree hygiene solution for your large-area environmental disinfection and vehicle preparation according to the European disinfection standard.

3. professional container hygiene services by state-certified disinfectors

Tailor-made H2O2 container disinfection processes for every requirement, no matter how dangerous and complex.

DIOP GmbH & Co. KG has been specialising for more than 15 years in the consulting, analysis and implementation of sophisticated disinfection processes in the fields of "Container preparation and ship disinfection". specialised. In this way, we make our customers' lives easier by guaranteeing them microbiological and legal safety (occupational safety for users with low chemical use in accordance with the Biological Substances Ordinance).

Advantages for you: Our disinfection service for container terminals from A to Z

  • Individual quotation calculation based on your specific, microbiological hygiene requirements
  • If required, joint pre-inspection (e.g. before annual validation, commissioning of vehicles, building complexes and rooms, after final building cleaning, in outbreak management, in pandemic situations as well as in emergency recovery plan or general emergency measures).
  • Detailed recording and logging of the disinfection measure with modern H2O2 measuring technology and hydrogen peroxide sensors
  • Decontamination work is carried out exclusively by experienced state-certified disinfectors and hygiene experts.
  • 6 log Validation by means of closed bioindicators and laboratory evaluation (if required, even accompaniment by external hygienist possible).
  • Free measurement of disinfection measures
Shipping container disinfection

The stress-free solution for complex disinfection of shipping containers

From the fields of maritime hygiene, pest control and container decontamination known from

Anticimex Disinfection
Cleanmarine Disinfection
Texbo Ship Cleaning
IHO List Disinfectants

The advantages for your hygiene and quality management

Automated disinfection processes according to the current state of the art (DIN EN 17272) significantly increase the efficiency of your container disinfection. Deficits caused by manual cleaning and surface disinfection processes are reliably compensated. 

Today, professional and serious hygiene, infection prevention and disinfection measures are regulated by authoritative European standards. Based on DIN EN 17272, we provide you with legal protection for your automated container disinfection processes. You are 100% on the safe side here. 

Complete and large-scale surface disinfection is naturally not possible with scrub-wipe disinfection, UV-C disinfection or ozonisation. Final H2O2 cold fogging without additional manpower, on the other hand, guarantees you enormous time and cost savings if you put the quality achieved and the effort involved in relation to each other.

Every user in your container and port terminal receives DIN EN 17272 certified in-house instruction training tailored to your needs by our experienced state-certified disinfectors.

 The topics of "process and occupational safety" and "accident prevention regulations" play a major role in the disinfecting reprocessing of containers. Therefore, you can also benefit from our disinfection process based on the principle of risk minimisation in the area of surface disinfection.

Well-known service providers, container ships and shipping companies have been successfully using DIOP's H2O2 cold fog disinfection for many years. You too can benefit from DIOP's standardised and validated hydrogen peroxide cold fogging.

Recommended by the

European Professional Association for Disinfectors

European Professional Association for Disinfectors

H2O2 container nebulisation in direct process comparison

Can automated cold fogging in accordance with EN 17272 of containers and container ships be used purposefully and optimally compared to other processes / active substances / disinfection technologies in the occupational safety concept of a container port and what are the advantages of such an airborne disinfection method for container processing ("Aerogenic Disinfection", "Airborne Disinfection Method")?

Advantages compared to other active substances and processes in the field of container disinfection measures:

  • Hydrogen peroxide fumigation

  • uv-C containerDisinfection

  • nebulisation sodium hypochlorite

  • Ozone container disinfection

H2O2 room fumigation versus H2O2 cold fogging

Advantages of hydrogen peroxide fogging compared to hydrogen peroxide room fumigation (gaseous hydrogen peroxide):

Process criteria

Hydrogenperoxide cold nebulisation (aHP process)

Hydrogen peroxide

Room fumigation

Validatable according to DIN EN 17272


(gram positive/negative)



Enveloped viruses

Unenveloped viruses



No high protein error

Little to no corrosion


No known disadvantages with

Micro aerosol technology

  • Expensive 
  • Time-consuming
  • Complex application
  • High concentrations
  • No effectiveness with biofilms and wetness

Microbiologically tested by

Dr Brill Steinmann
LS Laboratory AG
HygCen Austria
Research Circle of the Food Industry
Schwarzkopf Institute

What hygiene experts and users say

Hygiene expert office

pd dr andreas schwarzkopf

Specialist in microbiology and infectious disease epidemiology

In the field of surface disinfection in the healthcare sector, cold nebulisation processes for hydrogen peroxide have already been in use for many years. This type of disinfection can be integrated into your hygiene and disinfection plan without any problems.

Anticimex Disinfection

Maximilian p.

Anticimex GmbH & Co. KG

We are very satisfied with the MF Professional cold nebulisers!

Get free and non-binding advice now

Our team consisting of state-certified disinfectors is looking forward to the request of your container port or container terminal.

Which of our container disinfection solutions are you primarily interested in?
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  • Mobile container disinfection (portable device)
  • Mobile container disinfection (wheeled unit)
  • Disinfection as a service
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Frequently asked questions (FAQ) about decontamination of containers and shipping containers of all types and sizes:

Why do shipping companies, ships and container terminals increasingly rely on H2O2 cold mist disinfection as a safeguard for manual cleaning and disinfection processes?

A publication of the trade medium "viruses" Issue 13 in 2021, published by MDPI Verlag, sums up the added value of automated disinfection processes according to EN 17272 procedures in this context as follows:

Conventional disinfection methods are limited because they rely on the operator to ensure proper surface selection, product preparation and contact time of the disinfectant. "No-touch" room disinfectors reduce operator dependence and can thus improve the effectiveness of final disinfection [36]. Suspension tests for chemical disinfectants are not suitable for automated evaluation of airborne room disinfection [37].

Until now, such systems could only be tested on the basis of the French national standard NF T 72-281 (2014) [38]. However, in April 2020, the European Committee for Standardisation published the standard EN 17272:2020 for the evaluation of airborne room disinfection by an automated method (including the determination of bactericidal, mycobactericidal,sporicidal, fungicidal, levurocidal, virucidal and phagocidal activity) [39]. The recommended test viruses for the medical field are adenovirus and MNV [39].

Can DIOP's H2O2 cold fogging damage container materials, textiles, cargo or sensitive electronic equipment?

No, when our EN-certified disinfection process is used properly, there is no risk of damage to surfaces, packaging materials, boxes, container equipment, freight, textiles, sensitive electronics (even compatible with mainboards, circuit boards and micro-chips) or expensive medical equipment. This is impressively confirmed by more than 12 years of use at national and international level in more than 30 target groups. In addition, DIOP offers a guarantee for electronic and material compatibility. Please do not hesitate to contact our state-certified disinfectors at any time. A list of successfully tested materials, raw materials and electronic devices is available on request.

How long does the disinfection process take and how can it be validated?

The complete decontamination process by means of cold fogging from the start of the container disinfection measure to re-entering your container in question only takes between approx. 105 and max. 135 minutes (of which 60 minutes is the microbiological exposure time), depending on the on-site conditions. This makes our H2O2 fogging technology one of the fastest on the market for automated disinfection processes according to EN 17272. 

In the context of container reprocessing, the implementation of the disinfection measure is even faster, as shipping containers can be quickly and sufficiently ventilated via the large container doors directly after the 60-minute exposure time. This means that you can use your sea freight containers again without a protective mask and protective clothing after about 65 to 75 minutes, depending on the diosol concentration.

Since the fogging process has also been used for many years in the mechanical reprocessing of medical devices such as the interior of ambulances, incubators, intensive care units, etc., the good material compatibility has been proven and established.

You can conveniently carry out the validation yourself using microbiological bioindicators. Before decontamination, you place the biological indicators at the points to be checked and collect them again after completion of the measure. Together with the documentation sheet, you simply send the validation pack to the responsible microbiological laboratory for evaluation. You will receive the validation result after about 7 to 10 working days. Containers of the same or similar design do not all have to be validated separately, but you simply select one reference container per assembly / container model and thus save high validation costs.

Does the automated disinfection process meet all relevant standards for safe use in the field of container fumigation?

Yes, in addition to the authoritative European standard EN 17272:2020 for automated disinfection processes, we also comply with dozens of other EN standards for surface disinfection. The decontamination process also complies with the binding requirements of the Biological Substances Ordinance and the Hazardous Substances Ordinance with the respective associated technical rules (TRBA/TRGS) as well as the KRINKO recommendation "Hygiene requirements for the cleaning and disinfection of surfaces".         

Do I even achieve sporicidal disinfection (pharmaceutical requirement of 6 log) with H2O2 fogging equipment in addition to effectiveness against bacteria, fungi and viruses?

Of course, also in the context of container disinfection processes yes. Every disinfection measure can be validated container-related without any problems and in a user-friendly way. For this purpose, you can use our closed DioSpore controller bioindicators (BI), but also standardised bioindicators with sporicidal loading (6 log Geobacillus stearothermophilus or Geobacillus subtilis). Use our disinfection systems in combination with Diosol 12 or Diosol 19 (= special stabilised 19% hydrogen peroxide biocide).

The validation is considered passed if a reduction factor of Geobacillus stearothermophilus > 6 (SAL)
is achieved and there is at least 99 % difference
in RODAC plates in the before and after comparison (germ reduction).

What added value does automated disinfection provide if you have to clean your containers manually or disinfect them manually beforehand anyway?

Of course, cleaning / pre-cleaning before fogging is sensible and necessary because visible soiling on surfaces must be removed mechanically. However, according to the German Society for Hospital Hygiene (DGKH), the efficiency of subsequent disinfection measures is max. 70%. In containers, due to the large number and complexity of surfaces, the accessibility and effect of manual measures is certainly even lower. So there is a dangerous and unnecessary hygiene and disinfection gap of > 30% plus the already wipe-disinfected surfaces that have been treated with insufficient disinfection effect. Especially in the case of 3-MRGN or 4-MRGN as well as viruses, large-scale disinfection measures are necessary.

However, one of the biggest disadvantages of manual disinfection processes is the fact that they are NOT VALIDABLE and therefore the disinfection quality varies greatly from process to process (as it depends on the performance of the user). 

Similarly, the requirement for cleaning and final disinfection of surfaces cannot be fulfilled nearly without interruption by manual wipe disinfection: 

"Disinfection is intended to prepare the area/container/room/vehicle so that it can be used for transport without risk of infection. Depending on the potential contamination with pathogens, the final disinfection extends to all accessible surfaces and objects that are or may be contaminated with the pathogens."

Does the DIOP container disinfection process also work at freezing ambient temperatures in the container?

DIOP's aerogenous cold mist disinfection has been successfully tested between 4 and 45 °C to date. However, many containers often remain at freezing temperatures below 0 °C, depending on their geographical location, the time of year and the outside temperature. So what can you do to ensure effective container disinfection?

It'svery simple: Heat or warm up the container to at least 4 degrees Celsius using a mobile heating device. Then you are on the safe side in terms of microbiological performance. If the container is so cold that it represents a cold bridge due to the temperature, you can combine the fogging process with the heating process at the same time.

Otherwise, unnecessary condensation effects may occur in the container concerned.